cd8 apc cy7  (Cytek Biosciences)

Journal: bioRxiv

Article Title: Immune and Mutational Profile of Gene-Edited Low-Immunogenic Human Primary Cholangiocyte Organoids

doi: 10.1101/2025.01.20.628680

Figure Lengend Snippet: (a) Graphs showing percentage of positive mouse and human CD45 cells, percentage of human CD3, CD8, CD19 and human CD45 cell counts in weekly in peripheral blood (tail vein bleeds) for the two PBMC donor groups (PBMC Donor A, top; PBMC Donor B, bottom). Error bars represent mean±SEM of 4-8 mice per group.

Article Snippet: Humanization was evaluated weekly by flow cytometry of tail-vein bleeds collected in heparin-coated tubes (Sarstedt Ltd, 20.1309), followed by red cell lysis removal (StemCell Technologies, 07850) according to manufacturer’s instructions and using the following conjugated antibodies: mouse anti-human CD45-FITC (1:50, Thermo Fisher Scientific, 11-0459-42, clone [HI30]), mouse anti-mouse CD45.1-PE-Cy7 (1:100, Thermo Fisher Scientific, 25-0453-82, clone [A20]), mouse anti-human CD3-APC (1:50, Biolegend, clone [UCHT1]), mouse anti-human CD19-PE (1:50, Biolegend, 363004, clone [SJ25C1]), and mouse anti-human CD8-APC/Cy7 (1:50, Thermo Fisher Scientific, A15441, clone [RFT8]).

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Antibodies used for flow cytometry analysis and sorting of T cell populations from tumor and control bladder tissues.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques: Flow Cytometry, Staining

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Gating strategy for analysis of Tregs, Th cells, and CD8 + T cells ( A ). Immune cell infiltration measured as CD45 + out of all viable cells ( B ). Frequency of T cells and T cell subsets out of all CD45 + cells ( C – G ). Treg = regulatory T cells, Th = T helper, NMIBC = non-muscle-invasive bladder cancer, MIBC = muscle-invasive bladder cancer. Statistical significance was determined by the Kruskal–Wallis test followed by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Infiltration of CD45 + immune cells out of all viable cells and frequency of T cell populations out of CD45 + immune cells in tumor samples according to sex ( A – D , F , G ). Ratio of CD4 + /CD8 + T cells ( E ) Statistical significance was determined by Kruskal–Wallis test followed by two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Frequency of immune cells and T cell populations in tumor samples classified according to the Lund Taxonomy molecular subtypes. Infiltration of CD45 + immune cells among all viable cells ( A ). Immune infiltration in MIBC samples only ( B ). Percentages of T cells, CD4 + T cells, and CD8 + T cells out of all CD45 + cells ( C – E ). Ratio of CD4 + /CD8 + T cells ( F ). Percentages of Tregs and Th cells out of CD45 + cells ( G , H ). Statistical significance was determined through the Kruskal–Wallis test followed by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: T cell populations in tumor samples classified according to the Lund Taxonomy molecular subtypes. Percentage of T cells ( A ), CD8 + T cells ( B ), CD4 + T cells ( C ), Tregs ( D ), and Th ( E ) out of all viable cells. Statistical significance was determined by Kruskal–Wallis test followed by two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Transcriptional analysis of CD8 + T cells from non-malignant bladder tissue and from GU and Uro tumor biopsies. Principal component analysis ( A ). Heatmap of differentially expressed genes between CD8 + T cells from GU and Uro subtypes ( B ). GSEA using an exhaustion profile (Tirosh et al.’s 28-gene exhaustion profile) ( C ). Volcano plot with the differentially expressed genes (GU vs. control) marked in red, with the top 40 annotated ( D ). Volcano plot with the differentially expressed genes (Uro vs. control) marked in red, with the top 40 annotated ( E ). Percentage of CD8 + cells expressing the exhaustion markers CTLA-4, LAG3, PD-1, TIGIT, or TIM-3 measured by flow cytometry ( F ). Statistical significance assessed by Mann–Whitney U test.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Pathway analysis of differentially expressed genes between CD8 + T cells from GU subtype and the control ( A ) and Uro subtype and the controls ( B ). padj = 0.05 marked in red.

Article Snippet: APC-Cy7 Mouse Anti-Human CD8 , SK1 , BD biosciences.

Techniques: